Methods in Mammalian Embryology

A decolorant of the sulfhydryl reagent DTNB released from liver blastulae of sea urchins in saline medium.

The Development of Two Tailless Mutants in the House Mouse.

HE events that take place in the development of the mammalian T embryo have not been subjected to an extensive causal analysis so far. The reasons for this are“to be found mainly in the lack of suitable methods. It is not possible yet to use transplantation, isolation or vital staining techniques on mammalian embryos as they have been used on amphibian embryos. In the course of time it probably...

Novel Approach of Differential Staining to Detect Necrotic Cells in Preimplantation Embryos

Background This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst. MaterialsAndMethods Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were th...

A molecular basis for developmental plasticity in early mammalian embryos.

Early mammalian embryos exhibit remarkable plasticity, as highlighted by the ability of separated early blastomeres to produce a whole organism. Recent work in the mouse implicates a network of transcription factors in governing the establishment of the primary embryonic lineages. A combination of genetics and embryology has uncovered the organisation and function of the components of this netw...

Small group discussion for medical students to learning embryology

Background: One of the most important issues is the best method to teach embryology course to medical students. Small group discussion (SGD) were used to working together, integral to learningdeveloping intellectual skills and interactive learning experience. Methods: The 72 medical students were equally randomizedto the SGD (group I) and usual lecture based teaching (LBT), (group II) in genera...

Cultivation of Post-implantation Mouse and Rat Embryos on Plasma Clots.

INTRODUCTION D E S P I T E recent successes with the cultivation of mouse and rabbit eggs (references in Austin, 1961, pp. 144-7) techniques for the cultivation of post-implantation mammalian embryos have not hitherto advanced beyond those devised in the 1930's. Jolly & Lieure (1938) obtained development of rat and guinea-pig embryos explanted into homologous serum at stages between primitive s...